SrasV1, 2020, Analysis, bibRecord, 000A89

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

Identifieur interne : 000A89 ( 2020/Analysis ); précédent : 000A88; suivant : 000A90

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

Auteurs : Zheng Fan [République populaire de Chine] ; Yue Zhuo ; Xinyu Tan ; Zhi Zhou ; Jiangang Yuan ; Boqin Qiang ; Jinghua Yan ; Xiaozhong Peng ; George F. Gao

Source :

Journal of medical virology [ 0146-6615 ] ; 2006.

RBID : pubmed:16998888

Descripteurs français

English descriptors

KwdEn :
- Binding Sites, Cell Line, Humans, Nucleocapsid Proteins (metabolism), Protein Binding, SARS Virus (metabolism), Small Ubiquitin-Related Modifier Proteins (metabolism), Two-Hybrid System Techniques, Ubiquitin (metabolism), Ubiquitin-Conjugating Enzymes (metabolism).
MESH :
- chemical , metabolism : Nucleocapsid Proteins, Small Ubiquitin-Related Modifier Proteins, Ubiquitin, Ubiquitin-Conjugating Enzymes.
- metabolism : SARS Virus.
- Binding Sites, Cell Line, Humans, Protein Binding, Two-Hybrid System Techniques.

Abstract

SARS-CoV is a newly identified coronavirus (CoV) that causes severe acute respiratory syndrome (SARS). The SARS-CoV nucleocapsid (N) protein is an important structural and functional protein. To identify cellular proteins that interact with the SARS-CoV N protein and to elucidate the possible involvement of N protein in SARS-CoV pathogenesis, a human lymphocyte cDNA library was screened using a yeast two-hybrid system assay. hUbc9, a ubiquitin conjugating enzyme of sumoylation system, was found to interact specifically with the N protein, implying the post-translational sumoylation of the N protein. Mapping studies localized the critical N sequences for this interaction to amino acids 170-210, which includes the SR-rich motif. However, the consensus motif of sumoylation GK(62)EE in the N protein is not responsible for binding to hUbc9. Mutations of hUbc9 at the enzyme active site C93A or C93S severely impair the interaction with the N protein. The two proteins were also shown to colocalize in the cytoplasm of the transfected 293T cells. This is the first report demonstrating the interaction of hUbc9 with a structural protein of plus-strand RNA viruses, indicating a new drug target for SARS-CoV.

DOI: 10.1002/jmv.20707
PubMed: 16998888

Affiliations:

Links to Exploration step

pubmed:16998888

Le document en format XML

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<author><name sortKey="Yuan, Jiangang" sort="Yuan, Jiangang" uniqKey="Yuan J" first="Jiangang" last="Yuan">Jiangang Yuan</name>
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<term>Ubiquitine (métabolisme)</term>
<term>Virus du SRAS (métabolisme)</term>
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<front><div type="abstract" xml:lang="en">SARS-CoV is a newly identified coronavirus (CoV) that causes severe acute respiratory syndrome (SARS). The SARS-CoV nucleocapsid (N) protein is an important structural and functional protein. To identify cellular proteins that interact with the SARS-CoV N protein and to elucidate the possible involvement of N protein in SARS-CoV pathogenesis, a human lymphocyte cDNA library was screened using a yeast two-hybrid system assay. hUbc9, a ubiquitin conjugating enzyme of sumoylation system, was found to interact specifically with the N protein, implying the post-translational sumoylation of the N protein. Mapping studies localized the critical N sequences for this interaction to amino acids 170-210, which includes the SR-rich motif. However, the consensus motif of sumoylation GK(62)EE in the N protein is not responsible for binding to hUbc9. Mutations of hUbc9 at the enzyme active site C93A or C93S severely impair the interaction with the N protein. The two proteins were also shown to colocalize in the cytoplasm of the transfected 293T cells. This is the first report demonstrating the interaction of hUbc9 with a structural protein of plus-strand RNA viruses, indicating a new drug target for SARS-CoV.</div>
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SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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